Zhilin Yang

5 Questions 10 Answers 0 Followers

Questions related from Zhilin Yang

What is the blasticidin selection concentration for RPE-1 cells?

Hello Everyone I am performing a transfection of RPE-1 cell with some plasmid that contains blasticidin resistance. Anyone knows the concentration or range that I should use? Thanks!

08 August 2018 3,161 1 View

Which membrane of E. coli does FM4-64 stain?

I read two references regarding this question. Reference 1 says that FM4-64 majorly stains inner membrane. But reference 2 says FM4-64 stains outer membrane. Anyone knows how does FM4-64 exactly...

06 June 2017 7,517 5 View

Does bacteria move inside macrophage?

Hi. I have some questions about the movement of bacteria inside macrophage. 1) Anyone knows that whether bacteria will move around inside macrophage? 2)Also, once bacteria get uptake by...

04 April 2016 7,109 3 View

Is pBR322 origin compatible with f1 origin?

Hi. I'd like to insert two plasmids to E. coli strain, but 1. I wonder whether they are compatible. One plasmid is pTrc99A of which the origin is pBR322. And the other one is pASK-IBA3 plus, of...

01 January 1970 3,851 6 View

Which one has higher outer membrane permeability, MG1655 or BW25113

Hi Does anyone know which E. coli strain has higher outer membrane permeability, between MG1655 and BW25113? Thanks

01 January 1970 6,932 0 View