I want to determine protease and chitinase activity in several B. bassiana isolates and I am searching for a suitable method?
Spectrophotometry is a reliable and faster method for detection of enzyme activity. Accuracy depends on extraction and quantification.
In Silvaco, I have defined a semiconductor component. The electric field outputs have two problems. One is that the steps of X are not the same and they change, although I have defined a...
19 March 2024 4,649 0 View
The CASTEP in my case runs without materials studio. I want to calculate the band structure and optical properties of a perovskite material in this CASTEP code. I want to know how to write the...
28 February 2024 5,694 2 View
I want to develop a web application for some volunteer services that people can use it. but after people join that i want to use the data from information form and feedback forms for analyzing the...
08 February 2024 446 0 View
Improvement through visualization
22 November 2023 5,807 0 View
I'm facing challenges with the purity of plasmid DNA extracted from Trypanosoma brucei for PCR applications. Despite using a commercial DNA extraction kit, I suspect the presence of contaminants...
20 October 2023 829 3 View
Dear Sir or Madam I simulated a 3D PIN photodiode in SILVACO ATLAS. It stops in "material" and has an error: " Memory allocation failure. Try killing processes or see system administrator". There...
29 March 2023 7,621 3 View
I have done DFT calculation of a perovskite material (KGeI3) with CASTEP code. I want to find out its electron affinity, effective conduction or valance band density of states and relative...
17 March 2023 5,912 0 View
I study a reverse fault with several fault strands, each cutting different sediments (Holocene, Q3b, Q3, Q2). Am I allowed to sum each displacement related to each strand to get the cumulative...
27 February 2023 1,542 2 View
In the relevant electrochemical system, both Ox and Red species are electroactive and based on the scan rate equations, the mechanism is adsorption-controlled. According to the pH equations, the...
13 September 2022 6,568 0 View
I have some molecular weight data and I need a statistical definition for PDI to use as error bar.
12 September 2022 3,031 0 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?
17 July 2024 935 1 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...
13 July 2024 2,255 3 View
I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...
04 July 2024 8,100 4 View
Hi everyone! I have a little problem finding a method to quantify glucosinolates by uv-vis spectrophotometry without the use of Na2PdCl4. If anyone knows of any alternative methodology I would...
18 June 2024 9,961 0 View
I am conducting research on the antimicrobial synergism activity of lactobacillus strain with B.subtilis. in a recent experiment, we observed inhibition of B.subtilis growth with high...
01 June 2024 338 2 View
I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...
31 May 2024 6,444 1 View