Why do apoptotic proteins like PARP and caspases observe after 24 or 48 hrs while MAPK/PI3K pathways proteins observe after 1 or 2 hrs in vitro?

01 February 2015 4,520 5 View

Is there any extra care to culture 1975 cell line?

09 October 2014 444 4 View

Should the polycarbonate membrane be treated with collagen in a Boyden chamber migration assay for H460 lung cancer cells?

We are observing some chemotherapeutic drug effect in Boyden chamber migration assay using H460 lung cancer cell line and the following standard procedure. But we didn't observe any movement of...

06 July 2014 4,787 7 View

To isolate single lung interstitial cells from mice lung fibrosis models, which collagenase should be used?

I read some papers in which they used sigma C2139 (which is collagenase VIII) while some papers reported colagenase III. Can I use any collagenase to isolate interstitial cells for flow cytometry...

03 April 2014 681 3 View

For cytokine detection, is there any special treatment or method to detect IL-1B and IL-6 by using the dry method of western blot?

We are detecting different samples, tissues and cells, to detect inflammatory cytokines. We are getting good results for B-actin, NF-KB and IL-12 but after trying so many times we were unable to...

03 April 2013 1,527 17 View

I want to work on a mouse lung cancer cell line for the inflammatory response. Any suggestion which mouse(Not human) lung cancer cell line is the better choice?

I previously worked on the human lung cancer cell line A549 in BALB/c nude mice. Now I want to find out about the inflammatory response of some chemotherapeutic agent in BALB/c mouse by using a...

31 December 2012 4,638 2 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View