Noman Khan

7 Questions 8 Answers 0 Followers

Questions related from Noman Khan

Why do apoptotic proteins like PARP and caspases observe after 24 or 48 hrs while MAPK/PI3K pathways proteins observe after 1 or 2 hrs in vitro?

I read some papers they observe anticancer dugs effects using through western blot assay. They observe PARP or caspases or BAX or BCL-2 (apoptosis related protein) after long time point 24 or 48...

02 February 2015 4,556 5 View

Is there any extra care to culture 1975 cell line?

I got this cell line from 2 different sources. It primarily belongs to ATCC and culture according to ATCC guideline. But in both cases cells die after 2nd passage. I am using 10% FBS in RPMI media.

10 October 2014 485 4 View

Should the polycarbonate membrane be treated with collagen in a Boyden chamber migration assay for H460 lung cancer cells?

We are observing some chemotherapeutic drug effect in Boyden chamber migration assay using H460 lung cancer cell line and the following standard procedure. But we didn't observe any movement of...

07 July 2014 4,827 7 View

To isolate single lung interstitial cells from mice lung fibrosis models, which collagenase should be used?

I read some papers in which they used sigma C2139 (which is collagenase VIII) while some papers reported colagenase III. Can I use any collagenase to isolate interstitial cells for flow cytometry...

04 April 2014 715 3 View

For cytokine detection, is there any special treatment or method to detect IL-1B and IL-6 by using the dry method of western blot?

We are detecting different samples, tissues and cells, to detect inflammatory cytokines. We are getting good results for B-actin, NF-KB and IL-12 but after trying so many times we were unable to...

04 April 2013 1,571 17 View

I want to work on a mouse lung cancer cell line for the inflammatory response. Any suggestion which mouse(Not human) lung cancer cell line is the better choice?

I previously worked on the human lung cancer cell line A549 in BALB/c nude mice. Now I want to find out about the inflammatory response of some chemotherapeutic agent in BALB/c mouse by using a...

01 January 2013 4,676 2 View

Which mouse (not human) lung cancer cell line to use for checking inflammatory reaction of a drug?

Previously we were using A549 human lung cancer cell line in BALB/c mouse. Now we want to switch, mice cell line to human cell line. We need suggestions about which mice cell lines are a good...

01 January 2013 1,429 1 View