In the burgeoning field of epigenetics, there are several methods available to determine the methylation status of DNA samples. However, choosing the method that is best suited to answering a particular biological question still proves to be a difficult task.
Hi,
if you just need a global estimate of cytosine methylation I would recommend HPLC analyses. If you however need to understand patterns of cytosine methylation in a particular species, and would like to analyse divergence in methylation patterns or compare the methylation status of specific loci across samples then you need BS-seq (or MS-AFLP). See the attachment for further discussion
DNA Methylation Data Analysis Workshop
How to use bisulfite-treated sequencing to study DNA methylation
When?
12-15 November 2019
Where?
Berlin, Germany
Link?
https://www.ecseq.com/workshops/workshop_2019-07-NGS-DNA-Methylation-Data-Analysis
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