I am working on a research project to determine Environmentally Persistent Free Radicals (EPFRs) in wildfire ash samples by Electron Paramagnetic Resonance (EPR) Spectrometry. I already ran the samples and got the signals that confirm (based on g vale) the presence of free radicles and transitional metals (in some samples). I also do the baseline corrections as well. Now, which method should I use to develop the calibration curve and determine the concentration (spin/g) of free radicles? Should I use TEMPO or DPPH as standard?
From my previous trials on tissues using EPR, I think these references may be helpful. you can also find an excel sheet that may help you in the calculations.
good luck.
Rokyta, R., Stopka, P., Holecek, V., Krikava, K., & Pekárková, I. (2004). Direct measurement of free radicals in the brain cortex and the blood serum after nociceptive stimulation in rats. Neuroendocrinology Letters, 25(4), 252-258.
A. Abdel‐Rahman, E., Mahmoud, A. M., Khalifa, A. M., & Ali, S. S. (2016). Physiological and pathophysiological reactive oxygen species as probed by EPR spectroscopy: The underutilized research window on muscle ageing. The Journal of physiology, 594(16), 4591-4613.
Lund, A., Shiotani, M., & Shimada, S. (2011). Principles and applications of ESR spectroscopy. Springer Science & Business Media. (chapter 9, especially).
Swartz, H. M., Khan, N., & Khramtsov, V. V. (2007). Use of electron paramagnetic resonance spectroscopy to evaluate the redox state in vivo. Antioxidants & redox signaling, 9(10), 1757-1772.
Hi Nourhan S. Elkholy. I hope you are doing good. The excel sheet seems very helpful. Would you please give an insight or explain the parameters and the calculation little detail for me? That would be very helpful to me. One more thing, do you have any idea of converting free radicals concentration from M or ppm unit to spin/g? Thank you.
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