Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Cracked MEMS cantilevers despite wet release and critical point drying?

I have successfully released MEMS cantilevers with a photoresist sacrificial layer, using a dry plasma ashing technique. However, this process tends to damage the metal contact pads and result in...

15 November 2020 8,963 6 View

Hello Everyone, I am trying to regress a numeric variable on all categorical variables, which method would be the most suitable, ANOVA,Linear Regrsn?

I tried running Linear Regression but I feel the model has heteroskedasticity.

03 May 2020 6,874 6 View

When to use bounded Second Order Implicit Time Integration in Fluent?

Fluent offers different temporal discretization schemes. There are two implicit schemes: Second order and bounded second order. I am wondering what are the advantages and disadvantages of these...

12 December 2019 3,795 4 View

How to implement Distributed Activation Energy Model (DAEM) in ANSYS Fluent?

I want to model a chemical reaction in Fluent. However, reaction being too complicated, there is no single activation energy model available. The DAEM approach is used to estimate the products...

26 August 2019 7,144 1 View

How to model a chemical reaction in Fluent involving gaseous reactants and solid product?

I am currently simulating a system involving various chemical reactions. My primary phase is gaseous and secondary phase is solid. There are reactions involving solid to solid and gas to gas...

02 May 2019 3,826 3 View

Can I use k-epsilon turbulence model for transitional flow?

I have a geometry where the flow is transitional. i.e. a part of it has laminar flow and rest part has turbulent flow. Can I use k-epsilon model for this problem? Does it resolve the laminar...

28 March 2019 6,428 19 View

How we will evaluate the rocking response when a base isolated system is subjected to the rotational component of ground motion during earthquake?

There is a single mass system at base of which a base isolated system is attached. So this whole system is a two degree of freedom system which is subjected to rotational component of earthquake...

08 February 2019 962 1 View

How to get the diameters of droplets in Fluent?

I am simulating a diesel spray in air. I want to get the number of droplets and their diameters (preferably individual over mean) at certain time step. How can I extract this data?

13 December 2018 8,433 3 View

What are the advantages of Finite volume method (FVM) over Finite difference Method (FDM) for particularly flow simulation (CFD) ?

Most of the CFD solvers use FVM. However, FDM is pretty simple to implement. I am interested to know exactly what advantages do FVM offer. And when should one go for FDM if he is planning to write...

13 December 2018 8,641 38 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View