Which method is more efficient for producing multi-copy strain in Pichia pastoris, In-vitro multimerization or PTVA?

I am using pPICZalfa vector with gene length 1.1 Kb. Currently I am trying for In-vitro multicopy generation (Bgl II - BamHI), but the transformation efficiency in DH5alfa is decreasing as my...

11 January 2024 8,229 2 View