1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

Can the same primer pair , say for instance ITS 1 and ITS 4 generate different molecular weight amplicon after PCR for different fungal species?

Say Fungus A gives 500 bp and Fungus B gives 550 bp is it an absurd result?

29 December 2020 881 3 View

How to get nanocomposite of PEEK with Zirconia ?

Need a lengthy PEEK with Zirconia based nano composite of 180 mm length. Which method is possible? Tried injection molding no proper mix. Solvent casting process is also very costly. Kindly anyone...

13 December 2020 4,329 5 View

No title

30 November 2020 5,089 5 View

How can I get eggs of an univoltine casebearer moth for a threshold study?

I am planning to conduct a cage study to determine a critical density of an univoltine casebearer moth in red clover seed crop. This requires to release first instar larvae in the cages with the...

02 November 2020 6,577 3 View

Can anyone suggest me free and fast scopus publication with highly acceptance rate?

Q3,Q4 will be okay. I need scopus publication without APC and fast published. The paper is from Computer science discipline.

19 October 2020 4,255 4 View

Bio-intensive cropping systems can reduce the environmental foot print?

The adoption of bio-intensive cropping systems has the potential to reduce environmental footprints without compromising the economic gain per unit of resources.

12 October 2020 2,625 7 View

Application of Polyhalite changes the nutrients chemistry in soil?

Polyhalite is a multi-nutrient source, its application assume to be changes the soil biology and nutrients chemistry on long term basis

12 October 2020 6,685 3 View

If a certain paper cited is written language other than English, does its interpretation in another authors work become the baseline for it?

A paper not written in English , does it need to be read in its original form before being cited by anyone in the paper that they are writing . Or it is enough to view its interpretation in...

11 October 2020 4,280 8 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

Disposing Congo Red Agar?

Hi all, Hope you are well. My lab is planning to use some Congo red agar (0.01%) for Shigella flexneri, and we are wondering how do you dispose of the used agar plates. The Shigella contaminated...

13 January 2021 9,823 2 View

Oil Red staining AML-12, How much time did you wait?

Hi, Was wondering if any of you conducted an Oil Red on AML-12 cells, if so, after how much time did you executed this? Some studies call for 24hr and some for 18hr. For fat content analysis....

03 January 2021 8,613 1 View

Why below-ground ecology is still unexplored (majority of it) in our country ?

Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among...

02 January 2021 6,912 3 View

Why is my blot transfer bad now?

I had been having beautiful blot transfers, but in the last few days I have ran into issues where there is high background staining and poor band resolution. The background staining is very...

23 December 2020 670 3 View

Under what conditions absorption and excitation spectra of a same species can be different?

I took absorption and excitation spectra of a same species in the same solvent. Ideally I should get the excitation spectra almost similar to the absorbance spectra. But I got the excitation...

13 December 2020 8,965 2 View

Did anyone do microglia Oil Red O staining?

I need to stain adiposome in microglia, but I don't know how to confirm that are really success? method : Removed media and washed cells with PBS, then added 4 % paraformaldehyde for 20 minutes at...

24 November 2020 2,359 2 View

Is PBS a suitable vehicle for testing the hemolytic activity of drugs in Ex vivo red blood cell hemolysis assay?

I am working with small molecules with antiplasmodial activity. I want to evaluate the hemolytic activity using a regular assay assessing hemolysis by measuring the absorbance at 414 nm.

19 November 2020 7,573 3 View

Anyone an expert in algae in Victoria, Australia?

I found this (very pretty) algae (see picture) in a bore-fed and suburban catchment run-off fed, human made lake (Newport Lakes) in Newport, Victoria, Australia. I'm a vertebrate zoologist with a...

09 November 2020 7,587 3 View