We' re doing Pathscan Intracellular Signaling Array from Cell Signaling. It says positive control but not mention which one. Is this Beta actin or something? Thank you for your interest.
Ke-Wei Zhao
The positive spot on the slide can be found in the spec sheet. They are mostly the phosphorylated signal molecules themselves (e.g., P-Akt, P-Erk, etc). I have used one or two kits before and found that each slide contains 4 chambers/sets. You need to test different conditions to see what is optimal for your ligand (time, concentration, etc). So, plan carefully before using up the two slides.
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