Kindly share the lipid source you use in feed formulation for fish or shrimp.
Marine oils (e.g., fish, shrimp, krill), terrestrial plant oils (e.g., soy, peanut, sunflower), animal byproducts (e.g., fish and shrimp wastes, oils as a result of rendering poultry, pork and beef). What you use depends on the requirement of your species. Generally, marine fish and shrimp do best on marine oils rich in EPA and DHA.
It depends on you balanced n-3/n-6 or EFAs or not, If these fatty acids are balanced and there are some left of dietary lipid that you want to fulfill to reach the lipid level aimed. Any source of lipid can be used. In the same way, if you want to replace marine oil by plant oil, maximum replacement level should not impact balance of n-3/n-6 ratio or EFAs. Importantly, please check that how you fish or shrimp that you aimed to study utilize lipid well or not, if bad please control lipid level in diet in which provided optimum EFAs and the rest may be a little bit increase of protein or carbohydrate
This is depends upon the culture species for what you are formulating diet.
Mainly fish oil is preferable along with soy-lecithin as a source of phospholipid.
Since the less availability and high cost, the researchers incite to replace fish oil by using various vegetable oils.
But replacing fish oil using the plant oil (s) would affect the end product quality of cultured species.
The recent research suggested to switch over the species fed plant oil to fish oil containing diet (finisher diet) prior to harvesting for an appropriate time to restore both EPA and DHA as they are most important fatty acids in human nutrition.
A study with juveniles of P. monodon (Khan, 2013) shown that a period of 30 days has been required to restore both EPA and DHA by using a finisher diet (control diet with no fishmeal replacement) in both yard and laboratory condition, whereas finishing diet phase was 16 to 20 weeks in Salmon fed a blend of rapeseed, linseed and fish oil (Bell, Henderson, Tocher, & Sargent, 2004).
The variation in time phase between the studies could be due to the species difference, weight of the species, rearing and environmental conditions.
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