I want to estimate Non-starch polysaccharide content of various plant-based ingredients.I request you to please share the simple protocol that you are following to estimate the Non-starch polysaccharide.
Generally non-starch polysaccharides need to be determined by the exclusion principle (nonstarch PS=defatted dry mass - ash - starch - monosacharides -protein) - if you did not yet find the appropriate protocol, a link from a company called Megazyme provides an overview and also the detailed for the various analyses can be found: