Regarding biomass processing, I strongly recommend that you read this recently published article. About the transesterification process, this article may be enlightening: "Optimization of biodiesel production by in situ transesterification from dry biomass of Choricystis minor var. minor via response surface methodology"
Article A comparison of harvesting and drying methodologies on fatty...
In my view either microwave Pyrolysis or Hydrothermal Liquefaction is best for conversion of Microalgae to Biofuels and quickly we can convert. I think it may be cost effective. As I have attended one workshop at IIT madras in 2018 conducted by DR R Vinu and Dr Rajnish Kumar, you can contact the above professors through mail for further clarification.
20, what? mgKOH/g or %? Esterification cannot be skipped. I can only recommend our solvent born technique (Co-Sol), it can handle disorders associated to water content and provides high efficiency in processing
It greatly depends on the profile of triglycerides and fatty acids in oil being produced by your strain of microalgae. If it is really high in free fatty acids, then using alkaline catalyst without acid pre-treatment will be useless. On the other hand, using acid catalyst on a fresh oil could make it rancid. There are a lot of reviews on this matter and I am really happy to share some good articles if requested.