I am planning to perform a ELISA to quantify IgM, IgG and IgA against RBD SARS-CoV-2. I am going to use as positive control serum samples and bronchoalveolar lavage from mice infected with wild-type SARS-CoV-2. My question is: which inactivation procedure is the most effective to inactive the virus in order to carry out the experiment in BSL2 condition instead of BSL3 condition without altering antibody titers?

I have read that 56 degrees for 30 minutes reduce viral load but I am not sure is this is enough to work at BSL2 or if IgG, IgM and IgA levels can be reduced.