Since these are native peptides with no special modifications, I suggest you get some quotes for these synthetic peptides from commercial custom synthesis sources, along with quotes for peptide synthesizers. Used microwave peptide synthesizers go for between $25,000 and $50,000 US. You will find that you can buy hundreds of these peptides, in significant pure quantitates, for the same money it would cost to purchase and maintain a peptide synthesizer and the auxiliary equipment that would be required (i.e., UPS's for all the critical equipment, glassware and/or apparatus for cleavage from resin, HPLC for analysis and purification, ion exchange columns, rotary evaporator, and freeze dryer). If you are really going to be preparing hundreds of peptides over many years, or running a peptide core facility, then it is worth the investment of purchasing a peptide synthesizer. Also to consider are the time you have to invest in training people to use the instrument and all the auxillary instrumentation. Don't expect to share much of the equipment, in my experience many peptide preparations got ruined because other operators on shared equipment didn't know how to properly use the equipment or didn't care about others research. I often found that others released my frozen peptide preparations to atmosphere and allowed them melt back in the shared lyophilizer. Amino acid derivatives also cost money and some derivatives are not that stable; you would need to have the freezer space to store them in as well as space for the synthetic peptides you generate.

What scale will you be working at? How many residues are in the peptides? Are there any special chemistries required that would force you to use either t-BOC or Fmoc chemistry? If you have to use a t-BOC protection scheme are you planning to buy an HF apparatus to cleave the peptide from the resin or are you going be satisfied making peptides with an amide functionality at the c-terminus? These are all questions that need to be answered before I can make any specific suggestions. Generally I prefer to make peptides that are 10 residues or less using a manual synthesizer. Large scale solid phase synthesis (kg's of resin) I recommend using a CS Bio synthesizer. At a research scale I suggest purchasing a microwave assisted synthesizer from either CEM or Biotage.

Thank you for answering. I have no experience in peptide synthesis. I am looking for equipment which can be used for different common peptides. As example: Abltide (KKGEAIYAAPFA - NH2), Gly-Xaa sequences, Amyloid beta peptides, etc.

Since these are native peptides with no special modifications, I suggest you get some quotes for these synthetic peptides from commercial custom synthesis sources, along with quotes for peptide synthesizers. Used microwave peptide synthesizers go for between $25,000 and $50,000 US. You will find that you can buy hundreds of these peptides, in significant pure quantitates, for the same money it would cost to purchase and maintain a peptide synthesizer and the auxiliary equipment that would be required (i.e., UPS's for all the critical equipment, glassware and/or apparatus for cleavage from resin, HPLC for analysis and purification, ion exchange columns, rotary evaporator, and freeze dryer). If you are really going to be preparing hundreds of peptides over many years, or running a peptide core facility, then it is worth the investment of purchasing a peptide synthesizer. Also to consider are the time you have to invest in training people to use the instrument and all the auxillary instrumentation. Don't expect to share much of the equipment, in my experience many peptide preparations got ruined because other operators on shared equipment didn't know how to properly use the equipment or didn't care about others research. I often found that others released my frozen peptide preparations to atmosphere and allowed them melt back in the shared lyophilizer. Amino acid derivatives also cost money and some derivatives are not that stable; you would need to have the freezer space to store them in as well as space for the synthetic peptides you generate.

Thank you very much!

Have you tried looking at solution phase peptide synthesis ?

@Teodor

I have used older versions of peptide synthesizers from Protein Technologies (based in Tucson, Arizona, USA). I would recommend any of their synthesizers, but it would be prudent to evaluate which one best fits your needs and budget.

Synthesizers, Consumables, & Accessories

http://www.ptipep.com/products/peptide_syn/default.php

International Distributors

http://www.ptipep.com/services/distributors.php

From what I remember, the engineering and performance of Protein Technologies' synthesizers were very robust. The software interface on the Tribute was particularly easy to learn and interpret. Reaction progress and instrument status were clearly displayed on the Tribute's touch screen, plus the recorded UV-monitoring of Fmoc deprotection was a nice form of reassurance. Protein Technologies also provided software to predict difficult coupling reactions, which facilitated customization of protocols. Our technical support representative was especially responsive and helpful. Prior to purchasing, Protein Technologies invited one my colleagues to get hands-on product experience at their headquarters (not sure if they still do this). 

NOTES: I did mostly Fmoc-type solid-phase synthesis of 10- to 30-mer peptides (linear and cyclic). Although Protein Technologies has application notes for cyclizing and cleaving peptides on their synthesizers, I always did harsh or complicated reactions away from their synthesizers to minimize the risk of damaging them. (I transferred the resin-bound intermediates to traditional reaction vessels for steps involving TFA cocktails, expensive sidechain modifcations, or heavy metals.) Also, I packed my own amino acid tubes in order to "save money"—at least I improved my grip strength from crimping thousands of reagent tubes! The aforementioned synthesizers have substantial footprints, so lab space may become a consideration.

Hi Teodor,

We have the whole suite of peptide synthesisers (Biotage, CEM, Protein Technologies)  that we have used for the past 15 years. I would have to agree with Lael L Cheung in that the Protein technologies instruments ( we have 2 tributes and a PS3) have been the most reliable and easy to learn and set up. We found that once manufactures start adding "extras" like microwave (CEM, Biotage) performance goes downhill and you spend a lot of time troubleshooting.

And you also determine what sort of technical support is available and how close they are as all the synthesizers can make the ACP peptide (the test peptide) when the tech is present :-)

I have used PS3 from protein technologies. It's probably one of the cheapest and, for short peptides, might do the job. However, if you have the money, you should take a look at the microwave peptide synthesizers by CEM. We are now using a Liberty Lite, and it's just amazing. If you can afford the Liberty Blue, then it's even better.

http://cem.com/liberty-lite/

I have used CEM liberty as well as Liberty Blue they are much faster, less solvent consuming. So as suggested by previous researchers, if Money is not a problem, then CEM liberty blue is definitely best option to go with.

Hi Teodor,

I have used a syrII (Biotage, Multisyntech) for many years. It is stable and has made 1000s of peptides during the past 10 years. There is minimal maintenance and i have made peptides up to 72 aa and you have the opportunity to the synthesis at all steps. I can recommend either a syroI or II for standard Fmoc synthesis. The MW-synthesizers are also a good option but expensive and you can only make one peptide per synthesis - a syroII can make up to 48 peptides in 0.1 mmol scale..

Good luck finding the synthesizer for your needs

Søren  

Hi Mr Teodor,

Greetings

Better I suggest manual peptide synthesizer for short and ultra short peptides. Alternatively you can use microwave synthesizer for long peptides.

Good luck for SPPS

BIotage has some exciting products automated microwave. you can refer their website