I am trying to optimize a flow cytometry method to characterize mytilus spp. haemocytes. In literature I found that someone, to avoid clotting, use anti-clotting solutions or dilute and fix the haemolymph with formaline/paraformaldehyde while someone else just keep the samples in ice. According to my literature research mostly just use freshly haemolymph diluted with PBS buffer.
So I am wondering if the use of anti-clotting solution/fixing compounds will significantly improve the detection. Any tips?
Thank you all in advance!