Karina Hajdu

2 Questions 6 Answers 0 Followers

Questions related from Karina Hajdu

Can I digest my samples with SmaI at 25º and after digest with another enzyme at 37º?

Hi, I'm cloning a plasmid and want to confirm that the construct is correct. I had 3 options of dual-cutters (one cut inside my insert and one cut outside): ClaI, SpeI and NdeI. ClaI and SpeI...

07 September 2020 7,544 4 View

Tools for expression plasmid design?

Hello everyone. I am new to plasmid design and currently working with transposon/transposase systems (sleeping beauty and piggy bac). This is not so much a question but more of a request for...

01 March 2020 8,217 3 View