Primary monocyte-derived macrophages are resistant to infection. In fact, I have already tried to infect them with different serotypes of Adeno-associated virus expressing the GFP but without success.
What are the best methods to transfect or to transduce human primary macrophages?
Big Thanks
Dear Hamaidia,
The following publication covers the answer to your question:
Methods Mol Biol. Author manuscript; available in PMC 2010 Feb 13.
Published in final edited form as:
Methods Mol Biol. 2009; 531: 123.
doi: 10.1007/978-1-59745-396-7_9
PMCID: PMC2821576
NIHMSID: NIHMS172477
The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities
Xia Zhang, Justin P. Edwards, and David M. Mosser
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2821576/
Summary
Over the past three decades many techniques for expressing exogenous genes in a variety of cells and cell lines have been developed. Exogenous gene expression in macrophages has lagged behind that of other nonhematopioetic cells. There are many reasons for this, but most are due to technical difficulties associated with transfecting macrophages. As professional phagocytes, macrophages are endowed with many potent degradative enzymes that can disrupt nucleic acid integrity and make gene transfer into these cells an inefficient process. This is especially true of activated macrophages which undergo a dramatic change in their physiology following exposure to immune or inflammatory stimuli. Viral transduction of these cells has been hampered because macrophages are end-stage cells that generally do not divide; therefore, some of the vectors that depend on integration into a replicative genome have met with limited success. Furthermore, macrophages are quite responsive to “danger signals,” and therefore several of the original viral vectors that were used for gene transfer induced potent anti-viral responses in these cells making these vectors inappropriate for gene delivery. Many of these difficulties have been largely overcome, and relatively high efficiency gene expression in primary human or murine macrophages is becoming more routine. In the present chapter we discuss some of the gene expression techniques that have met with success and review the advantages and disadvantages of each.
Keywords: Adenovirus, DEAE-dextran, Electroporation, Lentivirus, Nucleoporation, Transduction, Transfection, Retrovirus, Vector
Hoping this will be helpful,
Rafik
The following link may provide you with certain valuable informations:-
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2821576/
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