Pritish Tidke

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Questions related from Pritish Tidke

How functional study effect when gene is fluorescently tagged endogenously by CRISPR knock-in or when it is over-expressed?

If I am studying the gene which is tagged by fluorescent tag endogenously by CRISPR/Cas9 Knock-in or if the same gene over-expressed and tagged with same fluorescent tag. Then what difference it...

06 June 2018 9,390 3 View

Which is the best suitable method for isolation of the GFP labelled cultured cells from the mixed unlabelled cells? FACS or serial dilution or other?

I want to isolate the GFP labelled clone from the mixed population of U2OS cell line, because some cells loosed the GFP expression, but they are antibiotics resistance. I am trying with serial...

01 January 2018 9,773 3 View

Whether Knock-in in hypertriploid U2-OS cell line affect insertion through CRISPR technology ?

I am planning for Knock-in in U2-OS cell line, which is chromosomally highly altered, with chromosome counts in the hypertriploid range. Hence whether the insertion of new gene into it cause any...

12 December 2017 8,660 2 View

How to transfect mCherry gene into the promoter of MDC1 gene by using CRISPR/CAS9 technology?

Hello Everyone, I want to generate transfected U2OS cell line , in which MDC1 will be fused with mCherry at its endogenous locus so mCherry will be under MDC1 promoter. How can I generate this...

11 November 2017 8,823 1 View