I guess the answer depends on how you define best: the strongest effect is probably seen in chemical or surgical models; if you however define best by most valid eg most closely resembling the human condition than other, more natural models may be better. Best regards, Thomas

Dear Parim,

I used Poloxamer-407 for the same. For detail protocol:

http://www.omicsonline.org/essential-oil-of-cymbopogon-citratus-against-diabetes-validation-by-in-vivo-experiments-and-computational-studies-1948-593X.1000098.php?aid=22036

http://benthamscience.com/journals/current-hypertension-reviews/volume/11/issue/2/page/143/

http://www.academicjournals.org/journal/JMPR/article-abstract/738A74423053

http://www.sciencedirect.com/science/article/pii/S1359511314005364

The use of animal models in diabetes research

Aileen JF King

 Abstract:Diabetes is a disease characterized by a relative or absolute lack of insulin, leading to hyperglycaemia. There are two main types of diabetes: type 1 diabetes and type 2 diabetes. Type 1 diabetes is due to an autoimmune destruction of the insulin-producing pancreatic beta cells, and type 2 diabetes is caused by insulin resistance coupled by a failure of the beta cell to compensate. Animal models for type 1 diabetes range from animals with spontaneously developing autoimmune diabetes to chemical ablation of the pancreatic beta cells. Type 2 diabetes is modelled in both obese and non-obese animal models with varying degrees of insulin resistance and beta cell failure. This review outlines some of the models currently used in diabetes research. In addition, the use of transgenic and knock-out mouse models is discussed. Ideally, more than one animal model should be used to represent the diversity seen in human diabetic patients.

Diabetes is a disease characterized by a relative or absolute lack of insulin, leading to hyperglycaemia. There are two main types of diabetes: type 1 diabetes and type 2 diabetes. Type 1 diabetes is due to an autoimmune destruction of the insulin-producing pancreatic beta cells, and type 2 diabetes is caused by insulin resistance coupled by a failure of the beta cell to compensate. Animal models for type 1 diabetes range from animals with spontaneously developing autoimmune diabetes to chemical ablation of the pancreatic beta cells. Type 2 diabetes is modelled in both obese and non-obese animal models with varying degrees of insulin resistance and beta cell failure. This review outlines some of the models currently used in diabetes research. In addition, the use of transgenic and knock-out mouse models is discussed. Ideally, more than one animal model should be used to represent the diversity seen in human diabetic patient.

Regards

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417415/

The above article should resolve most of your questions. I have worked with two 'natural' animal models of diabetes: the spiny mouse (acomys cahirinus) found in the Middle East and the tuco-tuco (ctenomys talarum) found in Argentina and perhaps elsewhere in S America. Both manifest insulin resistance. Let me know if you would like more details.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3417415/?report=reader

I guess the answer depends on how you define best: the strongest effect is probably seen in chemical or surgical models; if you however define best by most valid eg most closely resembling the human condition than other, more natural models may be better. Best regards, Thomas

You have got to be careful abt the type and strain of rodent model. That being said, you can use high fat, high fructose, dexamethasone among important models. There are genetic models as well. 

I do agree with Thomas

More natural will be the best models used.