I use sulfur content of elemental composition as a determining factor in differentiating pheomelanin from eumelanin. Can you suggest some more methods?
Almost all human melanins are a mixture of pheo and eumelanin. We have analysed many samples for pheomelanin and eumelanin by HPLC and electrochemical detection of AHP isomers (Kolb, A.M. et al ,Anal. Biochem. 1997) or by measurement of pyrole tricarboxylic acid after H2O2 or permanganate oxidation of eumelanin (Alaluf et al : Pigemnt Cell Res 2001). Original work on these methods have been published by Ito, S and Wakamatsu in many of their papers. We have also published some work on sulfur content in melanosomes by measuring sulfur using X-ray micro analysis (van Nieuwpoort, F. et al; J Invest Dermatol 2004 or other papers by Pavel, Smit, Koerten and van Nieuwpoort). The sulfur content by its own should be considered in the ratio to eumelanin or at least the total melanin content
Dear Kurian, please read Jonathan Rees paper
Eumelanin and pheomelanin concentrations in human epidermis before and after UVB irradiation. Jonathan Rees. He described how to measure and differentiate eumelanin and pheomelanin.
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