B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Mixed ANOVA independent variables understanding in SPSS?

I have an experiment where 10 groups of 3 judges are asked to evaluate a list of pictures in 2 evaluation conditions. The pictures were made in 2 between-subjects conditions (A,B) and each group...

20 December 2020 7,697 3 View

How to modelling the concrete rigid slabs in a masonry building ?

I am working on developing an accurate 3D model. When I defined the rigid slab the analyses aborted for the static step. In the Message file appeared this: ***NOTE: THE SOLUTION APPEARS TO BE...

22 July 2020 3,431 2 View

Any suggestion on how to solve problems on DNA gel electrophoresis?

Please find attached a photo of the problem; After a simple Agarose gel electrophoresis of DNA with EtBr i find the gel "divided" in two parts. Analizing at the gel at 259nm it seems that the...

28 April 2020 2,327 7 View

What kind of pharmacological / biological behavior is this?

14 April 2020 9,913 10 View

Can I use "all rights reserved" data for scientific use ?

Hi everyone, I am wondering if I can use "all rights reserved" resources (mainly texts) extracted from the web for testing my models and then (eventually) pubblish a paper. Notice that No data...

29 March 2020 5,026 4 View

Is there exist a tool which extract lexical and synctactical features from a raw text?

Is there exist a tool which extract lexical (e.g average word length, frequency of words) and synctactical features (e.g appositions) from a text?

09 March 2020 4,867 2 View

No title

05 February 2020 4,691 4 View

Harmonics analysis from a control theory perspective?

I was wondering if there are some references available on harmonics analysis (especially for power systems) from a control theory perspective.

08 December 2019 5,996 3 View

How can we conceptualise and explore the relationship between nationalism and climate change?

I have searched some of the top ranking nationalism studies journals (Nations and nationalism, SEN -Studies of ethnicity and nationalism, Nationalism and ethnic politics, Ethnicities,...

28 August 2019 1,344 13 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

What are the recently methods to estimate low-flow and high-flow time series in ungauged basins?

Hi Is there any new method to estimate ( predict ) low-flow and high-flow regimes in daily scales for ungauged hydrometric stations? Thanks

15 February 2021 3,493 3 View

What is the magnitude of yearly rhizopdeposition in tropical forests?

Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...

26 January 2021 4,278 4 View

Can you recommend a good book on analytical chemistry for non-chemists?

Dear colleagues, I am asking for you recommendation: I need a book on analytical chemistry in environmental sciences. As a non-chemist (a biologist) I have some basic chemistry knowledge but not...

25 January 2021 8,486 8 View

What is the best software for simulation of flow, sediment transport, and wave combination?

Dear coastal and river engineering researchers, I would appreciate some help from people who may be familiar with Flow, Sediment, and Wave related software with regards to the following: - Is it...

22 January 2021 6,024 14 View

How the microplastics levels in freshwater and marine water ecosystem be controlled?

Microplastics have been found in land, air, freshwater, effluent from a wastewater treatment plant and even in tap water and bottled water. The small plastic particles (less than 5 mm) pose a...

19 January 2021 8,637 3 View