Which is better ّ to measurement the plant extracts sensitivity,
agar diffusion method or disc diffusion method?
The method depends on the type of extract, the solvent, and the composition of the active ingredients in the extract. In general, the drilling method is preferred over the disc method, as it is easier and less prone to contamination.
Hi Dr
Agar diffusion method.
But depend on type of extraction and concentration.
If your extract is hydrophobic then disc diffusion may not perform well better do broth dilution methods. However no standards are developed for extracts and mm of diffusion is resistant, intermediate and susceptible, broth dilution followed by CFU gives good reproducible data, there are limitations with disk diffusion
Yes, Agar diffusion methods is suitable to used, please review the following link:
Article Agar Diffusion Method for the Assay of Colicins
Article Methods for in vitro evaluating antimicrobial activity: A review
Agar well diffuion method can be better than disc diffusion method.
