If I would like to introduce phosphate specific molecular imprinted polymers with a transducer to develop a phosphate biosensor, then which type of transducers can I apply. What are the different physico-chemical changes produces after binding of a MIPs with its target analyte?
Hi Nishant,
One type of transducer that I worked is quartz crystal microbalance (QCM). In QCM, a thin film of the polymer is deposited on the surface of a thin quartz plate, and the resonance frequency of the quartz crystal is measured. The frequency is decreased as the analyte molecules are absorbed or adsorbed onto the film.
As mentioned above, QCM is the best option for MIP based biosensor. Along with this you can try Electrochemical impedance (EIS) based electrochemical method for monitoring the changes in impedance, once your analyte binds to MIP.
Before looking for the best transducer to detect any specific binding I would firstly characterize the selectivity of the MIP binding by chromatography. Only if you got a sufficient selectivity for the matrix in which the sensor will be used, the search for the best transducer should start. During over 30 years’ experience in chemical sensors I have great doubts on the selectivity of MIPs as selective receptors.
Electrochemical or QCM.
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