Does Bromophenol Blue dissolve in water completely?

I am trying to make a 1% bromophenol blue solution by dissolving 100mg of the dye in 10 ml water. However, I am not getting a clear solution. I tried stirring it using a stir bar and also by...

09 February 2020 8,074 3 View

Is transient transfection good for protein expression?

I want to express and purify HCN1 channel protein. Will I get a good protein expression if I transiently transfect HEK cells with my channel expressed in a plasmid? This is all very new to me and...

07 August 2019 5,708 1 View

What is the volume of Strep-Tactin Superflow resin I should be using for protein purification?

I am using IPTG induction to express my protein of interest. At the end of induction and cell lysis, I'll be re-suspending my pellet in 40ml buffer. My protein has a N-terminus Strep-II tag and...

05 June 2019 4,568 3 View

What does low association and dissociation rate in surface plasmon resonance signify?

I am trying to understand the SPR graphs. Does low association and dissociation rate means the molecule has a low affinity kinetics for the substrate?

03 April 2019 4,397 1 View

If EF3/4 hands of KChIP is already bound to calcium how can they still be high affinity calcium sensors?

KChIPs belong to the NCS group of proteins and binds to calcium ions using their EF hands. Out of the 4 EF hands EF3 and EF4 are known as high affinity calcium sensors. However, my question is if...

09 October 2018 589 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View