I need to purify protein which will be further used for pull-down assay. My protein has a Strep-tag. Can anyone suggest a suitable column which will bind to Strep-tag and will enable purification of my protein of interest?
You can use any of these two for purifying Step-tagged proteins of interest from IBA Life sciences: Strep-Tactin® resin OR Strep-Tactin®XT beads.
https://www.gelifesciences.com/en/us/shop/chromatography/prepacked-columns/affinity-tagged-protein/streptrap-hp-p-00305
Hi,
You can use GE Strep Trap HP 1ml column for the purification. Also refer:
http://www.blossombio.com/pdf/products/UG_28913630AB.pdf
Good Luck.
Hi Ramyani,
Creative BioMart has a wide variety of chromatographic reagents, which you can try.
https://www.creativebiomart.net/Chromatography.htm
Hope it helps!
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