Ramyani De

6 Questions 3 Answers 0 Followers

Questions related from Ramyani De

Does Bromophenol Blue dissolve in water completely?

I am trying to make a 1% bromophenol blue solution by dissolving 100mg of the dye in 10 ml water. However, I am not getting a clear solution. I tried stirring it using a stir bar and also by...

10 February 2020 8,214 3 View

Is transient transfection good for protein expression?

I want to express and purify HCN1 channel protein. Will I get a good protein expression if I transiently transfect HEK cells with my channel expressed in a plasmid? This is all very new to me and...

08 August 2019 5,748 1 View

Which is a suitable column to purify protein having a Strep-tag?

I need to purify protein which will be further used for pull-down assay. My protein has a Strep-tag. Can anyone suggest a suitable column which will bind to Strep-tag and will enable purification...

06 June 2019 5,021 4 View

What is the volume of Strep-Tactin Superflow resin I should be using for protein purification?

I am using IPTG induction to express my protein of interest. At the end of induction and cell lysis, I'll be re-suspending my pellet in 40ml buffer. My protein has a N-terminus Strep-II tag and...

06 June 2019 4,626 3 View

What does low association and dissociation rate in surface plasmon resonance signify?

I am trying to understand the SPR graphs. Does low association and dissociation rate means the molecule has a low affinity kinetics for the substrate?

04 April 2019 4,442 1 View

If EF3/4 hands of KChIP is already bound to calcium how can they still be high affinity calcium sensors?

KChIPs belong to the NCS group of proteins and binds to calcium ions using their EF hands. Out of the 4 EF hands EF3 and EF4 are known as high affinity calcium sensors. However, my question is if...

10 October 2018 630 1 View