Hi Colleagues
Which immunoglobulin can pass through placenta ( specially in human), and why?
Hi Many cestodes can infect human through consumption their intermediate hosts, so which one can infect human when eating fish?
31 December 2017 8,318 6 View
Hi Please, if the add of extra primer (add 100 picomol/microliter instead of 10 pm/microliter ) can affect the positive results?
10 November 2017 3,719 5 View
Hi colleagues Please when I can use the touchdown protocol in PCR. Regards
10 November 2017 9,933 9 View
Hi Colleagues Please any one can provide me a sequence of specific primer of Theileria camelensis which infect camels or any related method for molecular identification of this protozoan parasite.
05 June 2017 1,284 14 View
Some time we need prepared Ag to detect some parasites serologicaly because these mainly not available commercially.
03 April 2017 7,350 8 View
Hi colleagues, how long I can preserve the specimens in glutaraldehyde for electron microscope examination.
01 February 2017 7,109 17 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...
14 July 2024 3,106 3 View