Hi Nataly,

I guess you can try following two kits from Sigma:

1. Glucose (HK) Assay Kit (#GAHK20)

2. Glucose (GO) Assay Kit (#GAGO20)

I have not used above kits. But I used Thermo Scientific™ infinity Glucose Hexokinase Reagents before, which shares similar approaches as Glucose (HK) Assay Kit from Sigma.

Regards,

Hua

I'm currently using one of Sigma's glucose kit (code GAGO20-1KT)to measure glucose, glycogen and trehalose in Drosophila adults. In order to measure glycogen you need to digest the glycogen to glucose using amyloglucosidase (A1602-25MG) and subtract your glucose result. Similarly, to calculate Trehalose you would digest your sample with trehalase (T8778-1UN). I'll attach a protocol for you

Thank you! 

It's much easier to buy Sigma kits here in Russia than Thermo Scientific, so I use them when possible. The problem was to chose one of these two.  

Hua uses tha HK version and Lewis - GO one. So I'd probably try both.

Lewis, thank you very much for the protocol, it's really helpful.

Hi, I just noticed I've made a couple of errors in the protocol I posted. One is to not include the recipe for the trehalase buffer

5mM Tris, 137mM NaCl, 2.7mM KCl – pH correct to pH 6.6. To make 100mL of a 10x stock you can use 606mg Tris, 8006mg NaCl, 201mg KCl

I also noticed that the concentrations for the trehalose standards are wrong. Per test they should be made up as 10µL as below and add 20µL of trehalase in buffer.

0.01 - 0.3µL trehalose+9.7µL buffer

0.02 - 0.6µL trehalaose+9.4µL buffer

0.04 - 1.2µL trehalose+8.8µL buffer

0.08 - 2.4µL trehalose+7.6µL buffer

0.16 - 4.8 trehalose+5.2 µLbuffer

Hi I have a related question, is there a problem if I read the absorbance at a different wavelength? I am asking because the equipment that I have don't have 540 but 560 nm.