I used to use Poly Ethylene Glycol 400 (Sigma) to dissolve the corticosterone and dilute the PEG to 10% with saline. PEG is an irritant, so you want to go easy on that. I prepared the CORT-PEG solution beforehand (left shaking once overnight (or until it was completely dissolved), afterwards at 4*C) and diluted it as needed.
There is a soluble form of corticosterone available as well, you could just dissolve it in saline.
Dr. Regina De Assis, thank you all for the answers provided to Nataly Yom Tov.
You did not tell us what volume of ethanol to what quantity of corticosteron.
Dear Vania R Assis,
Thanks a lot for your answer, I was wandering if you use the resulting solution for a single injection or if you can reuse this solution during a few days?
I see that you need to vortexed the solution before injection but for how long you can conserve this solution before the corticosterone crystal reform each other?
Regards,
Maeliss Hoarau
Vania R Assis I was planning to do subcutaneous injections and actually salin solution would be better for me to use. It has lower viscosity than oil and the pump I am using need viscosity lower than 20cp. I will check if it is possible to use the same dissolution than you did in saline.
Best,
Howdy, Hoarau Maeliss!
Did you ever have luck with the aforementioned procedure using saline in lieu of oil?
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