Untill now I was doing conventional PCR, there I was using ACTB as internal control and my gene of interest. 

Now I am planned to use real-time PCR for my gene of interest, where I am expecting the over expression of my gene of interest when I am treated with compound A. I am using comparitive delta delta Ct in ABI 7500 system. There I have to choose reference gene which should I use ACTB or my gene od interest