which factors do we need to consider while study of combining herbal extracts for analysis of Pharmacological screening?
Dear Sandeep Waghulde ,
Phytochemical screening involves qualitative and quantitative analysis of all bioactives (lipids, antioxidants, amino acids, sugars, minerals). Many factors must to take into consideration. For exemple, Methods to screening these bioactives varied a lot in the way of extraction and quantification.
Secondly, I will recommend the defatting (removal of lipids) of your raw plant sample using petroleum ether or hexane, after that when you prepare the extract it will more crystalline after lyophilization rather than oil nature.
Third, you can still dissolve the sample by performing ultra-sonication followed by providing temperature (45-55 *C) and filter your sample.
Please find an exemple in the attached file
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