It is difficult to find which fluorophores and quenchers are off-patent, i.e. in the public domain and free to use commercially without restrictions. Would anybody have a list of these (and the number of the original patent).
Their use on PCR probes might also be patented, so any information about this would be interesting.
In particular, I'm interested to know if FAM and HEX dyes are off-patent for PCR. I came across this site from IDT (https://eu.idtdna.com/pages/products/modifications/fluorophores/freedom-dyes), but unsure if these are dyes that are indeed in the public domain, or are dyes that IDT doesn't limit specifically.
Please read the following link :
http://www.amborella.net/2011-Graduate-PS/Week6-Microsatellite/Schuelke%202000.pdf
....trophoresis and a laser detection system, one of these primers has to carry a fluorescent dye label, which may be 6-carboxy-fluorescine (FAM), hexachloro-6-carboxy-fluorescine (HEX), 6-carboxy-X-rhodamine (ROX), or tetrachloro-6-carboxy-fluorescine (TET)2.
for the structure. The author wrote : fluorescin but it is perhaps a mistake . The right word is : fluoresceine.
Fluorescin is the dehydrofluoreceine name :
https://pdfs.semanticscholar.org/702b/828f0447b117c88fc8abfa6083bc17d04861.pdf
.. and visible. Rationale: These features would overcome the current limitations of
the fluorescin (colorless dehydrofluorescein)/fluorescein system while preserving
its advantages. Preliminary results from the PI's lab show that ..
If you have a friend in the chemistry dept.. , it can synthesize the wished dyes and purified them (It is a tedious work!!).
JRG
Please read this link as info for F.A.M
https://en.wikipedia.org/wiki/Fluorescein_amidite
Useful for further synthesis.
JRG
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