12 Questions 21 Answers 0 Followers
Questions related from Sebastian Vencken
I would like to link opposite 3' and 5' termini of a blunt-ended (optionally sticky-ended) DNA duplex without having to ligate stem-loop oligonucleotides to each end. This should result in two...
22 May 2017 8,085 9 View
It is difficult to find which fluorophores and quenchers are off-patent, i.e. in the public domain and free to use commercially without restrictions. Would anybody have a list of these (and the...
18 May 2017 7,810 3 View
Repetitive elements are very prolific throughout the human genome (up to 60% of the HG). SNPs are annotated by their position, not by their flanking sequences. I would be very interested in...
19 October 2016 9,218 1 View
I'm having some trouble obtaining an RNA 200-500 base fragment size in lysates from cells fixed with 1% formaldehyde. I've based the parameteres of my protocol on the Covaris protocol for Total...
24 November 2015 7,845 1 View
I'm producing ~200 nm siRNA PLGA/DOTAP nanoparticles using the double-emulsion solvent evaporation technique. When I sized the particles using DLS the majority (95%) were of the correct size...
30 June 2015 2,734 8 View
I would like to isolate bronchial epithelial cells from human bronchial brushings and am exploring various options. I simply want to deplete any other cell types and there's no need to culture the...
22 August 2014 2,697 14 View
I'm aiming to attempt the opposite of what many on this forum are trying. I'm seeking to perform Trizol extraction with the implicit aim to purify RNA species that are longer than small RNA...
14 February 2014 9,212 2 View
Seeking information on Reversible RNA-RNA crosslinking
10 July 2013 9,976 2 View
I'm performing a RNA-precipitation protocol with specific biotin-TEG labelled probes which bind streptavidin magnetic beads. Over time my enrichments have become significantly lower. My suspicion...
12 June 2013 3,213 1 View
I've come across various methods to determine the summarised error in qPCR experiments that included all biological and technical replicates. Although I haven't had much trouble performing these...
26 May 2013 3,918 26 View
Hi, I'm analysing data from a Affymetrix GeneChip Gene 1.0 ST microarray experiment. I'm analysing the data with the RMA method with the mixed model, nested ANOVA for the analysis of differential...
15 October 2011 4,416 1 View
I'd really appreciate it. http://www.ncbi.nlm.nih.gov/pubmed/2438234 http://www.ncbi.nlm.nih.gov/pubmed/3204101 Many thanks
25 August 2011 363 1 View
