Sebastian Vencken

11 Questions 20 Answers 0 Followers

Questions related from Sebastian Vencken

Can the ends of the same DNA duplex be covalently linked without using stem-loop oligos?

I would like to link opposite 3' and 5' termini of a blunt-ended (optionally sticky-ended) DNA duplex without having to ligate stem-loop oligonucleotides to each end. This should result in two...

22 May 2017 8,048 9 View

How do I find SNPs in repetitive sequences?

Repetitive elements are very prolific throughout the human genome (up to 60% of the HG). SNPs are annotated by their position, not by their flanking sequences. I would be very interested in...

19 October 2016 9,178 1 View

How can I shear RNA from formaldehyde-fixed cell lysates with Covaris M220?

I'm having some trouble obtaining an RNA 200-500 base fragment size in lysates from cells fixed with 1% formaldehyde. I've based the parameteres of my protocol on the Covaris protocol for Total...

24 November 2015 7,810 1 View

Secondary size peak for PLGA nanoparticles. Why?

I'm producing ~200 nm siRNA PLGA/DOTAP nanoparticles using the double-emulsion solvent evaporation technique. When I sized the particles using DLS the majority (95%) were of the correct size...

30 June 2015 2,704 8 View

How can I isolate bronchial epithelial cells from bronchial brushings?

I would like to isolate bronchial epithelial cells from human bronchial brushings and am exploring various options. I simply want to deplete any other cell types and there's no need to culture the...

22 August 2014 2,662 14 View

Anyone have experience with trizol (phenol:chloroform) extraction while excluding small RNAs?

I'm aiming to attempt the opposite of what many on this forum are trying. I'm seeking to perform Trizol extraction with the implicit aim to purify RNA species that are longer than small RNA...

14 February 2014 9,178 2 View

Are there any reagents that can produce inter-RNA crosslinks that are reversible without having to introduce special nucleotides to the RNA?

Seeking information on Reversible RNA-RNA crosslinking

10 July 2013 9,947 2 View

Biotinylated oligonucleotide probe degradation?

I'm performing a RNA-precipitation protocol with specific biotin-TEG labelled probes which bind streptavidin magnetic beads. Over time my enrichments have become significantly lower. My suspicion...

12 June 2013 3,182 1 View

qPCR: Standard deviation of reference sample?

I've come across various methods to determine the summarised error in qPCR experiments that included all biological and technical replicates. Although I haven't had much trouble performing these...

26 May 2013 3,853 26 View

Microarrays: P-value or FDR

Hi, I'm analysing data from a Affymetrix GeneChip Gene 1.0 ST microarray experiment. I'm analysing the data with the RMA method with the mixed model, nested ANOVA for the analysis of differential...

15 October 2011 4,382 1 View

Could anybody please send me the following two papers.

I'd really appreciate it. http://www.ncbi.nlm.nih.gov/pubmed/2438234 http://www.ncbi.nlm.nih.gov/pubmed/3204101 Many thanks

25 August 2011 334 1 View