Several parameters are evaluated in order to determine the most efficient protein ratio in the feed for fish larvae, Researchers have focused on different enzymes in several studies. Which enzyme activities must be taken into account for such study?
Dear Tolga Şahin
Digestive enzymes are classified based on their target substrates:
Proteases and peptidases split proteins into small peptides and amino acids.
Lipases split fat into three fatty acids and a glycerol molecule.
Amylases split carbohydrates such as starch and sugars into simple sugars such as glucose.
Nucleases split nucleic acids into nucleotides.
Good luck
Dear Tolga,
The three main categories of enzymes which are responsible for digestion of the principal food components are:
1- Proteases for protein.
2- Lipases for lipids.
3- Amylases for carbohydrates.
You can choose any single member of one category with others from other categories. However, you can focus on specific enzymes according to the food habits of your fish. Predatory or carnivorous fish mainly consume protein rich diets, so the primary working enzymes are proteases. In contrast, plant eating fish or herbivorous mainly consume carbohydrate rich plant materials, so the most active enzymes will be amylases. The same is applicable for lipases according to fat proportion in food. The final options will depend on the ultimate goal of research; nutrition, physiology or growth for example, to choose any specific enzyme to study.
Best wishes
SM Najim
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