I have a protein source with varying results of amino acid profile. For instance; when the sample is hydrolysed with "6 M HCI"
L-Valine level is 0.24g/100g whereas it is 3.08g/100g when hydrolysed with "3 M p-TSA" . And the requirement of the animal is 1.50g/100g.
On the contrary when hydrolysed with "6 M HCI" the L-Leucine level is 7.99g/100g while hydrolysed with "3 M p-TSA" it is only 4.00g/100g.
If the sample is used as the only protein source, which criteria should I take into consideration when choosing one from the results?
According to the available literatures, only 6M HCl is used most frequently to hydrolyse the protein for amino acid analysis.
Thx for the answer Dr. Jannathulla,
In the literature, 6M HCl is the most common method for hydrolyse. We choose this method due to the reason that there is HCl for breakdown of proteins into smaller molecules in fish stomach I guess. So, even though we get higher levels of aminoacids when hydrolysed with "3 M p-TSA", we do not take this result into account and specify as useless, am I right?
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