I am trying to do segmentation for breast cancer organoids to see the difference of HER2 surface expression between the cells after IF staining. In order to do the segmentation I would stain the cytoplasm and us it as segmentation marker. any ideas?
Dear Nooraldeen Tarade,
You may want to look over the following data:
https://emulatebio.com/organ-chips/?utm_feeditemid=&utm_device=c&utm_term=organoids&utm_source=google&utm_medium=ppc&utm_campaign=**LP+-+NonTM+-+General&hsa_cam=13979478193&hsa_grp=125257935299&hsa_mt=p&hsa_src=g&hsa_ad=534955409440&hsa_acc=8851317517&hsa_net=adwords&hsa_kw=organoids&hsa_tgt=kwd-325079228858&hsa_ver=3&gclid=CjwKCAiArOqOBhBmEiwAsgeLmSyRbPsu5WzraSR_5QseDuwRzluIJ3xILBxq-_ZQ9X295ejUfXB-5RoCEk0QAvD_BwE
https://www.nature.com/articles/s41578-021-00279-y
Dear Nooraldeen Tarade,
Yet, look at the following data:
https://www.sakuraus.com/Products/Advanced-staining/Tissue-Tek-Genie-Advanced-Staining-System.html?utm_source=google&utm_medium=cpc&utm_campaign=genie_P
https://biotium.com/technology/cellular-stains/?gclid=CjwKCAiArOqOBhBmEiwAsgeLmQlsHOeb6mcZVPyrq-Jvyv2p2gFredA59z92yYkTVF37H8vuRqHcMxoCXCQQAvD_BwE#mitochondria?keyword=mitochondrial%20stain&creative=560959318506
This may be helpful
https://www.thermofisher.com/order/catalog/product/D3911
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