Nooraldeen Tarade

3 Questions 4 Answers 0 Followers

Questions related from Nooraldeen Tarade

Which cytoplasm stain you would use for organoids?

I am trying to do segmentation for breast cancer organoids to see the difference of HER2 surface expression between the cells after IF staining. In order to do the segmentation I would stain the...

09 January 2022 2,557 3 View

Does Paraformaldehyde fixation affects the antibodies binding in immunofluorescence (IF) ?

I am doing IF staining for intracellular domain of HER2 receptor. Sometimes I keep the cells in 4% PFA for 1-2 weeks in the fridge till I stain them. Does this affects the antibody biding affinity?

13 October 2021 4,268 11 View

MCF10A infection failure ?

I have a problem in infection step for MCF10A by Lenti virus 3ed generation ( VSVG , GAG-pol and PWZL.hygro H-Ras G12V), it did not work ,but it works with Lenti lox GFP ? Could you please suggest...

05 October 2017 5,718 0 View