Which cell viability assay would you recommend for human oral cancer?

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How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

30 July 2024 1,412 8 View

How to determine the quantity/amount of secondary phase based only on peak intensity?

I have 3 different specimens. Each specimen has a main peak of the targeted phase, however, all of them also have a secondary peak of associated with a secondary phase. Now just based on the...

26 June 2024 5,049 5 View

How can machine learning be employed in the field of natural sciences?

Hello! I am pursuing Ph.D. in Environmental Sciences from Sambalpur University, India. My thesis topic broadly focuses on physico-chemical analysis of water and soil samples. I eagerly want to...

18 June 2024 1,897 4 View

Can someone help me with the alpha amylase inhibition assay?

I am determining the alpha amylase inhibition activity of my methanolic extracts by DNSA method. The problem is, my sample absorbance is higher than the negative control which made the final...

27 May 2024 9,348 0 View

How can I find permission and access to parenting style questionnaire?

I need to use and adapt the parenting style questionnaire in my ongoing research project, can somebody please help me regarding gaining permission and access to them as I am unable to find the...

25 May 2024 6,304 1 View

Cell Culture Debris? or Contamination?

I'm doing OSCC cell culture and routine media (supplemented MEM) changes every 72 hours. The media looks healthy in terms of color (reddish-orange), and there was no microbial contamination...

12 May 2024 9,279 2 View

How to improve RNA Integrity in bacteria using Purelink RNA mini kit?

I am using Purelink RNA mini kit (Fischer Scientific) to extract total RNA from bacteria. i need to get better RNA integrity number like more than 7, but for only one sample I got 7.4, for other...

01 May 2024 4,355 3 View

How to simulate Wall shear stress in COMSOL?

Water flows in a 2D pipe, I am able to prepare a simple simulation resulting in the velocity and pressure across the pipe. But I am interested to simulate the Wall shear stress generated by the...

22 April 2024 8,537 5 View

How to achieve stable quantum computations?

Achieving stable quantum computations is a challenging task due to the fragile nature of quantum systems. Welcome your valuable answers please. I am also proposing an idea to achieve this as...

21 April 2024 10,032 1 View

PhD candidate with a research focus on chronic inflammation particularly macrophage cells so i need professor information this area??

I am a PhD candidate in immunology at AWKUM with a research focus on chronic inflammation, particularly the role of macrophage cells. I am writing to express my keen interest in your research on...

28 March 2024 7,798 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View