generally in the process of agarose gel electrophoresis, TE buffer is used as the tank buffer which facilitates the movement of the DNA molecules from the negative terminal to the positive terminal
TAE is often used for larger fragments of dna ( 12kb and larger)and TBE is quicker for smaller dna fragments.TAE has less buffering capacity and may need recirculating for over 6 hour runs. TAE is better for recovering dna for downstream use. TBE has greater buffering capacity but the DNA moves a little slower TRis phosphate buffers are used but purification of the dna for some downstream purposes involving phosphorylation are a problem.Alkaline NaOH/EDTA buffer is used for single stranded dna separation