Which bacterial conjugation system is most widely conserved?

More Drew Hansen's questions See All

When designing a plasmid with an oriT site, does it matter how much space is flanking the sequence if there is a nearby promoter?

I have a promoter downstream of my gene of interest, and I am wondering if the conjugation proteins would interfere with the transcription of that gene.

10 November 2017 1,709 0 View

What is the usefulness of adding a transcription terminator to a plasmid?

What are the downstream effects of using these terminators?

29 October 2017 7,859 4 View

What makes a good genotype of an E. coli strain for conjugation?

Are there any specific mutations in the host chromosome that will make the system better to study?

29 October 2017 5,557 1 View

Which bacterial conjugation system is most widely conserved?

I've seen common conjugation systems use RP4, R388, R1, F plasmid, etc but which system is most related across every conjugation system? Which proteins are found in most/all of the conjugation...

29 October 2017 4,644 3 View

What are the steps to designing the strains and plasmids in a conjugation experiment?

How do I choose the right strain of E. coli for the donor and recipient? Which genes need to be included on the conjugative plasmid besides an antibiotic selection marker? How does this change for...

13 October 2017 9,096 2 View

What is the usefulness of adding a transcription terminator to a plasmid?

What are the downstream effects of using these terminators?

09 October 2017 2,875 4 View

What are the steps to designing the strains and plasmids in a conjugation experiment?

How do I choose the right strain of E. coli for the donor and recipient? Which genes need to be included on the conjugative plasmid besides an antibiotic selection marker? How does this change for...

09 October 2017 5,286 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What are the key methods and indicators used in assessing the biodiversity of river ecosystems, and how do these methods account for variations ?

Biodiversity assessment of river ecosystems is crucial for understanding the health and stability of these environments. This question aims to explore the various techniques employed to evaluate...

07 August 2024 4,290 3 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Michael J. Benedik

The conjugation system belongs to the plasmid, not to the host. So asking which is conserved across the most species doesn't really make sense. If you want to be more precise about what you are asking, there might be someone who can answer.

Benjamin THOHA Thomas

Your question is not correct. Kindly rephrase again, because conjugation system belong to the plasmid and not the host

Drew Hansen

You're both correct, sorry about that. I've changed the question.