What makes a good genotype of an E. coli strain for conjugation?

Drew Hansen @Drew_Hansen

30 October 2017 1 6K Report

Are there any specific mutations in the host chromosome that will make the system better to study?

Mostafa Haghpanah

dear Drew Hansen

this article can be helpful

https://link.springer.com/protocol/10.1385/1-59259-409-3:61

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When designing a plasmid with an oriT site, does it matter how much space is flanking the sequence if there is a nearby promoter?

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