Drew Hansen

8 Questions 3 Answers 0 Followers

Questions related from Drew Hansen

When designing a plasmid with an oriT site, does it matter how much space is flanking the sequence if there is a nearby promoter?

I have a promoter downstream of my gene of interest, and I am wondering if the conjugation proteins would interfere with the transcription of that gene.

11 November 2017 1,738 0 View

Which bacterial conjugation system is most widely conserved?

I've seen common conjugation systems use RP4, R388, R1, F plasmid, etc but which system is most related across every conjugation system? Which proteins are found in most/all of the conjugation...

30 October 2017 4,676 3 View

What is the usefulness of adding a transcription terminator to a plasmid?

What are the downstream effects of using these terminators?

30 October 2017 7,890 4 View

What makes a good genotype of an E. coli strain for conjugation?

Are there any specific mutations in the host chromosome that will make the system better to study?

30 October 2017 5,589 1 View

What are the steps to designing the strains and plasmids in a conjugation experiment?

How do I choose the right strain of E. coli for the donor and recipient? Which genes need to be included on the conjugative plasmid besides an antibiotic selection marker? How does this change for...

14 October 2017 9,122 2 View

What are the steps to designing the strains and plasmids in a conjugation experiment?

How do I choose the right strain of E. coli for the donor and recipient? Which genes need to be included on the conjugative plasmid besides an antibiotic selection marker? How does this change for...

10 October 2017 5,335 2 View

Which bacterial conjugation system is most widely conserved?

I've seen common conjugation systems use RP4, R388, R1, F plasmid, etc but which system is most related across every conjugation system? Which proteins are found in most/all of the conjugation...

10 October 2017 4,919 3 View

What is the usefulness of adding a transcription terminator to a plasmid?

What are the downstream effects of using these terminators?

10 October 2017 2,903 4 View