19 September 2023 3 10K Report

Looking for an HPLC method to quantify them

More Luigi Tucci's questions See All
How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to stain bacterial surface for electron microscopy?

I would like to investigate the surface of bacteria using electron microscopy to assess changes in specific conditions. Is there an established protocol for that? Thank you.

15 July 2024 2,338 2 View

How to assess bacterial permeability?

Is there an established protocol to measure the permeability of bacterial membranes? Thank you

30 June 2024 6,614 3 View

How to immobilize bacteria without morphology alterations?

I have a set of bacteria that I stained with fluorescent dyes to check for viability. The protocol suggests to place 5 ul of the suspension on a slide, cover with a cover glass, and observe on a...

16 June 2024 9,382 1 View

How to transfect environment bacteria?

I would like to transfect bacteria with some plasmids. However, the bacteria are not competent but either isolated from the environment or purchased reference strains. Can I transfect these...

16 June 2024 9,384 6 View

How to describe statistical values in words?

After performing statistical analysis, there is the need to report the p-values in the text. Is there an accepted (published) stratification range? For instance, is 0.04 'slightly' significant?...

28 May 2024 7,724 3 View

How to assess if Kd values (constant of dissociation) are meaningful?

I used Microscale thermophoresis(MST, from Nanotemper) to determine the constant of dissociation (Kd) between a protein (20 nM) and a ligand (1:2 serial dilution from 50 μM to 1.5 nM). I ran the...

04 May 2024 2,066 0 View

How to stain live bacteria for real time tracking?

I would like to track in real time the growth of bacteria using fluorescent miscoscopy. I remember there was at least one plasmid that could be used: transfected bacteria would emit a red or green...

29 April 2024 7,095 3 View

What magnification for organoid imaging?

Hello, I would like to take some publication-quality pictures of organoids. What magnification shall I use? Do I need a 60x or is a 40x enough? Thank you

15 April 2024 8,471 1 View

How to sterilize pipette without autoclave?

Hello, I think I am using a 5 mL pipette that is contaminated with bacteria. Nothing serious, just the usual environmental microbes. The problem is that the tips for the 5 mL pipette are without...

07 April 2024 6,716 1 View

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I could've sworn that I read a protocol where strained plant "juices" were added to agrobacterium cultures in lieu of pure acetosyringone. If you have heard of anything like this, please let me...

06 May 2024 9,001 0 View

What is the chances of getting microorganisms in packed apple juices found inside Kathmandu?

I am researching on it and i didn't find any articles published on this topic

13 January 2024 312 2 View

Do you use the hardness of pomegranate peel to determine the harvest date?

The hardness of the peel of pomegranate fruits is affected by irrigation, the environment, and the number of fruits on the tree, so it is difficult to rely on the hardness of the peel in...

10 January 2024 3,609 1 View

What is the formula for commercial beet sugars and invert sugars in sugar beet?

For analysis of sugar beet juice quality, what formula is used to calculate commercial beet sugars and invert sugars

27 November 2023 5,721 0 View

Techniques for characterising and screening green-skinned grapes and their derivatives?

I have grown some green-skinned grapes with seeds (based in UK), I am looking to develop some food analytical science research activities around characterising and screening these grapes and their...

17 November 2023 8,307 0 View

How to produce bioethanol from sugarcane juice?

I want know about pretreatment on sugarcane juice for fermantion and either yeasts generat eith microorganisms or externally added dried yeast.

15 July 2023 1,727 0 View

Is it possible to carry out ultrasound assisted extraction of juice samples in ultrasonic bath and how?

How to carry out ultrasound assisted extraction of commercial juice samples in ultrasonic bath.

03 June 2023 5,789 0 View

Could you suggest a practical way of sodium bicarbonate consumption in order to enhance sports performance?

Sodium bicarbonate is among five evidence-based performance supplements (caffeine, creatine, nitrate/beetroot juice, β-alanine, and bicarbonate). I need a pragmatic approach to the culinary use or...

13 March 2023 2,342 2 View

Application of deep learning for fruit masking and classification?

In one of the projects with the help of python (jupyter notebook), I applied mask RCNN for masking pomegranate fruit and classify it based on its size. During model application in real time the...

07 February 2023 7,987 3 View

Which possible phytochemicals will i get if i will use n hexane as a solvent for extraction?

The plants I'm using are Polyathia longifolia, Saraca Asoka and peels of pomegranate

25 January 2023 8,735 4 View

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