How to carry out ultrasound assisted extraction of commercial juice samples in ultrasonic bath.
Basically, i am trying to understand from the literature whether lobbying can also considered an alternative source of financing fiscal deficit.
26 July 2024 8,691 5 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,591 5 View
I have a plant whose roots have been infected with both bacteria and fungi. What strategy should I follow to isolate RNA of all three, i.e. plant, bacteria and fungi at once? This RNA is to be...
09 June 2024 9,876 0 View
if I want to run my battery cycling test in 1 C rate ,but then I need to know the capacity of my material used in the coin cell (i.e. if my capacity is 20mAh then I will put a constant current of...
05 May 2024 1,364 1 View
I'm currently experiencing convergence failure while attempting to optimize a dimeric liquid crystal molecule under the influence of an electric field. The optimization code I'm using is...
10 March 2024 4,737 0 View
implying that a molecule with a lower dipole moment exhibits lower hyperpolarizability and polarizability compared to one with a higher dipole moment?
07 March 2024 572 2 View
Im trying to run dbscan.py by using vmd (dcd and pdb) files but the script is showing error. Its not generating cluster it's only generating noise from the trajectory file. How to solve this issue...
03 March 2024 6,414 0 View
I am conducting a study that involves analyzing categorical outcome variables with multiple levels and want to employ multinomial logistic regression analysis in SPSS for my data. However, I'm...
29 February 2024 8,533 4 View
Hello everyone, I am currently working on developing a method to quantify butyric acid in plasma on single quad-GCMS using electron impact mode. For this purpose, I am currently following this...
14 February 2024 3,919 0 View
What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes withou using ready to use lymphocytes seperating medium?
29 January 2024 3,222 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 946 1 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,827 0 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View