Which are the main limitations of SPR comparing with BLI, especially in terms of buffer and matrix especially for liposome-protein interactions assays ?
Ciao Francesco,
I can give you some tips about the difference between SPR and BLI :
-If you are working with precious material, your sample could be reuse with BLI.
-BLI is easier to use if you don't have an extended knowledge in SPR.
- About the buffer, there is no difference, maybe except the DMSO %.
- Unspecific binding could be observe with liposomes. So, different types of SPR sensorchips could be tested ( limited diversity of BLI biosensors).
Vanessa
Thanks for your answer, regarding data quality is SPR way better then BLI according to your experience ?
@francesco
SPR gives reproducible and accurate data. You can monitor and interprete the signal binding. However, BLI is easier to use and give you a good answer in a quick time and with less materials.
Best
Vanessa
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