Can I use carbo-free blocking solution in place of normal goat serum?

I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...

07 February 2021 7,520 6 View

Can problems with blocking cause high background and weak antibody signal during immunofluorescence staining?

I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...

10 November 2020 3,620 7 View

Mask for outdoor exercise during covid pandemics?

Hi !! I've seen a lot of arguments in favour and against the use of a mask while running outdoors. I think it's just that some people are not comfortable enoughwearing one, but maybe there is some...

08 July 2020 4,927 2 View

Help editing citantion style in Mendeley. Multiple paper by the same authors?

I'm trying to edit an existing citation style to suit my needs (Journal of Tropical Ecology). I edited it so the inline citations go from newest to oldest reference (Smith et al 2019, Jones et al...

15 June 2020 9,926 4 View

How to measure ratio/amount of PCR products?

Hello, I am trying to look at the efficiency at which a Gene is cut after tamoximen treatment. Thus we got a set of primers that can tell us weather the Gene is cut or not. We wanted to measure...

02 February 2020 7,599 3 View

Why GFP PCR in E. Coli is not about 50% 50% with respect of GFP expressing bacteria and non-GFP expresing bacteria?

As you can see, most of our colonies express GFP while we expected to get only 50% of these or less.

04 December 2017 8,378 3 View

Bumpy melting curves in qrtpcr? I am having some extra picks in my melting curves. I think is primer dimer since it is in NTC?

I am getting some extra bumps in my qrtpcr for actin. I see it in the nyc and in the x10*5 standard. So I think is primer dimer forming when there is not enough template. I tried adding more cDNA...

05 November 2017 5,899 7 View

What causes smearing in western blots?

I ran a 15ug western blot using protein from treated mammalian cells. The top bands are Ligase 4 and the bottom bands are B-Actin. I am not sure why there is smearing in the Ligase 4 bands but not...

12 September 2017 3,730 2 View

Whole cell fatty acids from hydrocarbon soil samples

05 June 2017 6,186 0 View

Don't know what statistical analysis to use?

Does Intrinsic vs. Extrinsic Motivation Play a Role in People who Exercise Alone or With a Partner? The independent variable for this experiment is whether they exercise with a partner or not. The...

21 May 2016 8,266 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View