if we can design the short base-pairing ssDNA by the 3', 5' and middle of Aptamer, and testing the DNA statue aftter adding the target, such as arsenic?
colorimetric and resonance scattering (RS)-based biosensor for the ultrasensitive detection of As(III) in aqueous solution via aggregating gold nanoparticles (AuNPs) by the special interactions between arsenic-binding aptamer, target and cationic surfactant. Aptamers and the cationic surfactant could assemble to form a supramolecule, which prevented AuNPs from aggregating due to the exhaustion of cationic surfactant. The introduction of As(III) specifically interacted with the arsenic-binding aptamer to form the aptamer–As(III) complex, so that the following cationic surfactant could aggregate AuNPs and cause the remarkable change in color and RS intensity.
The binding of the arsenic aptamer is controversial. Check this paper for reference:
https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b02789
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