Hello,
I'm looking for antibodies to stain lipid droplets in fixed cells. I can see the lipid droplets in cells using the lipid markers (LipidSpot and NileRed), but I would like to see it also using antibodies. Anyone has some suggestions, which antibodies are good for such experiment?
Thank you,
Sarka
Hi! This would depend on the cell type under study, as the LD protome may differ between different cell types. Typically perilipin family proteins are highly abundant LD proteins but which perilipin to use would depend on the cell type, using Human Protein Atlas will help. kindly, Veijo
Hello .. you can use Antibodies for nay of the well-known LD-associated proteins such as Perilipin, TAP etc. You have to select the candidate of the particular protein depending upon the system that you are probing.
What cell types are you using? PLIN2 is a nearly ubiquitiously expressed lipid droplet that is often used as a lipid droplet marker. We have used this antibody with success to visualize PLIN2 in U2OS cells - Rabbit polyclonal anti-Plin2 Abgent Cat. # AP5118C; RRID: AB_10662954.
In regards to James' comment, I would still check first: https://www.proteinatlas.org/ENSG00000147872-PLIN2/cell as there are in fact quite many cell lines where PLIN2 is not expressed or very low. For example, in A431 cells which we have been using we have not been able to detect it by any antibody.
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