Hi, just a short thought: Helminths usually initiates a Th2 type immune response. The C57Bl/6 strain is not a very good Th2 responder strain in comparison to other inbred strains such as Balb/cJ or NIH/Ola, which I have also worked with in allergy studies. (Allergy is also Th2 response). For example - I used the Th2-promoting adjuvant aluminiumhydroxide, but the antibody response was much higher in the two latter strains compared to the C57Bl/6-strain. So as Frederic Beaudoin points to, it is very important to consider the type of immune response you want to study and from this select strain and adjuvant. Best regards, Jitka

Hello

Try with Filariae and Echinococcus multilocularis

References :

http://app.egyptlearn.com/eji/pdf/june2010/10-Sherif%20Abdin2.pdf

https://www.jstor.org/stable/3276138?seq=1#page_scan_tab_contents

Undo

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC98736/

http://shodhganga.inflibnet.ac.in/bitstream/10603/31108/7/07_chapter2.pdf

Good Luck 

Thanks for your kindness Saleh Alkarim.

Thank you dear Touraj ... good suggestion.

Adjuvants are made to favor different kind of immune response. Multiple factors are to be taken into account when selcting them. First of them is of course the type of immune response needed. Are you trying to protect the mice against your helminth? if so, you have to know the type of immune response that ;lead to protective immunity.  You could also only need antibody to different antigens of your crude extract, then the type adjuvant needed may possibly be different. Dont forget that the type of mice and the mode of administration are important to the type of immune response that will be elicited in your animal model.  Finally don't forget that crude extract by itself will be immunogenic and that too strong adjuvant could lead to necrosis.  The best would be to do a preliminary study with 2-3 mice in each group and to test different types of adjuvants.  

Hi, just a short thought: Helminths usually initiates a Th2 type immune response. The C57Bl/6 strain is not a very good Th2 responder strain in comparison to other inbred strains such as Balb/cJ or NIH/Ola, which I have also worked with in allergy studies. (Allergy is also Th2 response). For example - I used the Th2-promoting adjuvant aluminiumhydroxide, but the antibody response was much higher in the two latter strains compared to the C57Bl/6-strain. So as Frederic Beaudoin points to, it is very important to consider the type of immune response you want to study and from this select strain and adjuvant. Best regards, Jitka

Frédéric Beaudoin and Jitka Stilund Hansen,

really thanks for your complete answer to my question. I hope using your brilliant experiences during my research.

Best wishes.

C57 mice are sensitive to show modulation of immune response by adjuvants. compared to other strains of mice, such as Balb/c or CD-1. C57 strain of mice are able to distinguish the Th1 or Th2 types of response by evaluation of IgG subclass response, showing development of IgG2a antibodies in addition to IgG 1 with adjuvants that modulate the immune response towards Th1 type and producing predominately IgG1 response with adjuvants modulating the immune response towards Th2 type.

For Th1 type of response one of the best adjuvant to be used is Advax"CpG". 

I suggest that you may try poly I:C. It can help to elicit balanced immune responses, including both cellular and humoral responses. And this also has been proved by a recently published paper in PLOS ONE.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145637

Thank you Mr "Rajesh K Gupta" and Mr "Yanmin Wan" for your kind replies.

Hi,

For induction of protective immune response against a pathogen, there are three parameters, 1- adjuvant, 2- nature of vaccine  3- route of immunization. You should select your adjuvant according the immune platform you needed. CFA for induction of strong Th1 response, Alum for strong Th2 response and Montanide ISA 206, 266, 720 for both Th1/Th2 responses.