Dear Jai Krishna Mahto:

it is not clear if you are thinking about full scale application of bioremediation or about laboratory testing and research. For full-scale application commonly it is not allowed to use pathogenic strains. It is regulated by legislation of a given country or state concerning human health protection and soil protection.

No legislation allows application of pathogenic bacterial strains for any purpose (bioremediation, plant protection, .....) without protection of human health and environment.

Regards

Vit

no

Hello,

You may use pathogenic bacteria but no further than you get rid of the pathogenesis factors that these bacteria carry. In case of Pseudomonas aeruginosa not every strain is pathogenic. For example, P. aeruginosa PAO1 is on BSL2. It is more of an opportunistic pathogen, and it is not as pathogenic as e.g P.aeruginosa PA14. There are several ways to deal with pathogenes. One is knock-out of pathogenesis factors, another is taking genes which you are interested in, creating vector and then transformation of nonpathogenic strain. Both ways are unfortunately difficult and quite expensive if you do not have external funding for your research. 

Good luck!

Although it is not clear but still I am having a P. aeruginosa strain showing better PGPR activity. So, as suggested by Adrian after getting rid of the the pathogenesis factors you may try for their application in bioremediation purpose.

Good luck

No.There are bacteria with the potential for degradation of this molecule that are not pathogenic andand can be applied in bioremediation processes . Try to isolate other bactéias that present no risk to humans and the soil is the basis for the production of safe food.

Dear Jai Krishna Mahto:

We cannot utilize the pathogenic bacteria for bio-remediation. On the other hand, the genes which are responsible for bioremediation that was cloned and used for bioremediation. For example In Pseudomonas aeruginosa as responsible for heavy metal resistance like cadmium that gene (cadR, cadA) was cloned into non-pathogenic bacteria and used for bioremediation process. This way is possible. This agreed with Pierlorenzo Brignoli answer.

Thank You

For full-scale or field application you won't be allowed to use pathogenic strains having bioremediation potential. It is also legally regulated/prohobited if there is possibility of affecting human health and environmental harm. There is possibility of identifying the gene responsible for heavy metal resistance (e.g. for cadmium resistance in Pseudomonas aeruginosa responsible genes (cadR, cadA) may be cloned into non-pathogenic bacteria and could be used for bioremediation process.